液相色谱-二极管阵列检测器/离子阱质谱法检测水中的5种微囊藻毒素

摘要

A method for the determination of five microcystins (MCs) in water was established using liquid chromatography ( LC )-diode array detection ( DAD )/ion trap mass spectrometry (IT MS). The five MCs in water were enriched and purified by solid phase extraction, and determined by LC-DAD/IT MS. DAD was used for both qualitative and quantitative analysis,and IT MS was used for qualitative analysis only. Under the optimized conditions, the detection limits of 5 MCs in water were 0.1 μg/L. The average recoveries of the three spiked levels (0.2,0. 8 and 5 μg/L) were 52.2％ - 115.2％ with the relative standard deviations (RSDs) of 1.2％ -10. 0％. This method uses UV absorption spectrum and MS spectrum in the qualitative and quantitative analysis, and can be applied in the determination of various MCs in both surface water and drinking water.%建立了一种液相色谱-二极管阵列检测器(LC-DAD)/离子阱质谱(IT MS)对水中5种微囊藻毒索(microcystins)的分析方法.水中的微囊藻毒素经固相萃取富集和净化,经LC分离后,采用DAD和IT MS定性分析,DAD定量分析.在优化的条件下,水中5种微囊藻毒素的检出限为0.1 μg/L;3个质量浓度加标水平(0.2、0.8和5 μg/L)的平均回收率为52.2%～115.2%,相对标准偏差为1.2%～10.0%.该方法从紫外吸收光谱和质谱角度同时进行定性定量分析,可用于地表水和饮用水中多种微囊藻毒素的检测.