高效液相色谱法同时测定食品接触材料中抗氧化剂和紫外吸收剂的迁移量

摘要

采用高效液相色谱技术，建立了食品接触材料中多种抗氧化剂和紫外吸收剂迁移水平的检测方法。该方法测定的23种目标化合物具有较好的线性关系，相关系数（ r2）≥0.9998，检出限和定量限分别在0.01到0.22 mg / L 之间和0.03到0.85 mg / L 之间。依据欧盟指令（ EU）No．10/2011，考察了5种食品模拟物30 g / L 乙酸、10％（v / v）乙醇、20％（v / v）乙醇、50％（v / v）乙醇和油类模拟物（异辛烷）中抗氧化剂和紫外吸收剂的迁移量。该方法回收率在92.8％~117.7％之间，相对标准偏差在0.95％~9.72％之间。探讨了不同实验条件对抗氧化剂和紫外吸收剂回收率的影响。结果表明，该方法准确、稳定，完全满足欧盟指令（EU）No 10/2011和 GB 9685-2008对食品接触材料及制品中抗氧化剂和紫外吸收剂特定迁移量（ SML）的限量要求，并利用该方法测定了30批次食品接触材料中抗氧化剂和紫外吸收剂的迁移水平。%An efficient analytical method for the quantitative determination of migration levelsof antioxidants and ultraviolet absorbents in food contact materials by high performance liquidchromatography( HPLC) has been developed. The analytical method showed good linearitywith the correlation coefficients( r 2)≥ 0. 999 8 for all the compounds. The limits of detectionwere between 0. 01 mg / L and 0. 22 mg / L and the limits of quantification 0. 03 were in the range of to 0. 85 mg / L for the 23 analytes. According to the European Union Directive No 10/ 2011,five food simulants were investigated:30 g / L acetic acid,10%( v / v)ethanol,20%( v / v)ethanol, 50%( v / v)ethanol,and fatty food simulant( isooctane). The recoveries were in the rangeof 92. 8% -117. 7%,with the relative standard deviations of 0. 95% -9. 72%. The effects of differentexperimental conditions on the recoveries of antioxidants and UV absorbents were studied.The results showed that the method is accurate and stable,and can meet the requirements ofEuropean Commission Regulation( EU)No 10/ 2011 and GB 9685-2008 for the specific migrationlimits( SML)of the antioxidants and ultraviolet absorbents in real food contact plastic materialsand article samples. The method has been applied to determine the migration levels ofantioxidants and ultraviolet absorbents in different simulants from the migration tests of 30 bat ches of food contact material samples.