配对使用陈皮半夏对颈动脉粥样硬化家兔磷脂酰肌醇3激酶和磷酸化蛋白激酶B表达的影响

摘要

Objective To investigate paired using tangerine peel and ternate pinellia tuber to regulate phosphatidylinositol 3-kinase (PI3K)and protein kinase B (p-Akt)signal pathways in rabbits in order to treat carotid atherosclerosis. Methods According to a random number table,20 rabbits were divided into 4 groups:a control,a model,a tangerine peel and ternate pinellia tuber,and a tangerine peel and ternate pinellia tuber+LY294002 (a PI3K/Akt signal pathway specific inhibitor)group (n=5 in each group). The rabbits in the control group were fed with basal diet,while those in other groups were given high-cholesterol diet and air drying of carotid artery intima were performed. After procedure,the rabbits in the tangerine peel and ternate pinellia tuber group and the tangerine peel and ternate pinellia tuber +LY294002 group were treated with the decoction of traditional Chinese medicine. Carotid atherosclerosis was observed via HE staining;the effect of tangerine peel and ternate pinellia tuber on the expressions of PI3K and p-Akt in rabbits with carotid atherosclerosis was observed by western blot. Results (1)the expressions of PI3K and p-Akt in carotid in the model group were 107. 0 ± 2. 6 and 113. 0 ± 1. 7,and those in the tangerine peel and ternate pinellia tuber group were 174. 7 ± 14. 5 and 186. 3 ± 18. 3. There were significant differences between the two groups (P <0. 01). After using PI3K/Akt signal pathway specific inhibitor LY294002,the expressions of PI3K and p-Akt (117. 0 ± 4. 0,127. 3 ± 4. 7)were lower than the tangerine peel and ternate pinellia tuber group. There were significant differences between the two groups (P <0. 01). (2)The tangerine peel and ternate pinellia tuber group had mild intimal hyperplasia and foam cell formation under the artery intima,however,they were better than those of the model group and the tangerine peel and ternate pinellia tuber+LY294002 group. Conclusion Tangerine peel and ternate pinellia tuber may play a role in the treatment of carotid atherosclerosis by upregulating the expressions of PI3K and p-Akt in vascular endothelial cells.%目的：探讨配对使用陈皮半夏对家兔磷脂酰肌醇3激酶（PI3K）-蛋白激酶B（Akt）信号通路的调节而治疗颈动脉粥样硬化。方法取20只家兔，通过随机数字表法分为对照组、模型组、陈皮半夏治疗组和陈皮半夏＋LY294002（PI3K/Akt通路特异性抑制剂）组，每组5只。给予对照组家兔基础饲料喂饲，其余各组予以高脂饲料并实施颈动脉内膜空气干燥术。术后对陈皮半夏治疗组和陈皮半夏＋LY294002组家兔使用中药灌胃。通过 HE染色，观察颈动脉粥样硬化的病理学改变；通过Western blot方法，观察陈皮半夏对颈动脉粥样硬化家兔血管PI3K和p-Akt表达的影响。结果（1）颈动脉内PI3K与p-Akt蛋白表达相对量，模型组为107.0±2.6和113.0±1.7，陈皮半夏治疗组为174.7±14.5和186.3±18.3，两组比较差异有统计学意义（P＜0.01）；使用PI3K/Akt通路特异性阻滞剂 LY294002后，颈动脉血管内皮的 PI3K和 p-Akt表达（117.0±4.0、127.3±4.7）低于陈皮半夏治疗组，两组比较差异有统计学意义（P＜0.01）。（2）造模后4周，病理检查显示兔颈动脉内膜轻度增生程度陈皮半夏组轻于模型组。结论陈皮半夏有可能通过上调血管内皮细胞中PI3K和p-Akt的表达，发挥治疗颈动脉粥样硬化的作用。