EGFR反义寡脱氧核苷酸硫代修饰片段调节人肝癌细胞中E-cadherin mRNA表达的研究

摘要

Objectives：This study was designed to investigate modulation of phosphorothioate oligodeoxynucleotides(S-ODNs) of EGFR on E-cadherin mRNA expression in human hepatocellular carcinoma cells. Methods：Treated with concentration of 3.2 μ mol/L S-ODNs in culture medium， the relative E-cadherin mRNA level at 84 hour in human hepatocellular carcinoma cells was evaluated by reverse-transcriptase polymerase chain reaction with using HPRT (hypoxanthine phosphoribosyltransferase) as an internal control standard. Result：The expression of E-cadherin gene mRNA was enhanced obviously by S-ODNs. Compared with control group, the relative expression level of E-cadherin gene mRNA was increased at about 66.36％ (P<0.05). Conclusion： S-ODNs is the regulator of E-cadherin gene expression, it can enhanced E-cadherin gene expression in human hepatoma cells. Understanding the regulation of E-cadherin gene expression in human hepatocellular carcinoma cells might contribute to development of a new preventive and therapeutic strategy for tumor invasiveness.%目的：研究表皮生长因子受体（epidermal growth factor receptor，EGFR）反义寡脱氧核苷酸硫代修饰片段对人肝癌细胞E-cadherin基因表达调节。方法：应用逆转录聚合酶链反应（RT-PCR）方法。以HPRT(hypoxanthine phosphoribosyltransferase)为内标，分析3.2μmol/L浓度的EGFR反义寡脱氧核苷酸硫代修饰片段在作用84h时对人肝癌细胞基因E-cadherinmRNA表达水平的影响。结果：EGFR反义寡脱氧核苷酸硫代修饰片段有明显的刺激人肝癌细胞表达E-cadherin的作用，与正常对照组相比，其E-cadherinmRNA表达水平增高约66.36％（P<0.05）。结论：EGFR反义寡脱氧核苷酸硫代修饰片段是人肝癌细胞表达E-cadherin基因的调节剂，能刺激人肝癌细胞E-cadherin基因的表达;本研究为预防和治疗肝癌的侵袭转移提供了新的途径和方法。