毕赤酵母的密码子用法分析

摘要

通过分析Pichia pastoris的28个蛋白编码基因的同义密码子使用情况并计算该酵母的密码子用法，首次确定出P.pastoris的19个高表达优越密码子。这些结果经与已知的Saccharomyces cerevisiae及Kluyveromyces lac-tis的密码子用法基本相似，但在氨基酸谷氨酸的密码子选择上截然相反，提示这可能属于P pastoris所偏爱的密码子用法。%According to the synonymous codons used in 28 open reading frames from Pichia pastoris, the codon usage in this species was calculated and 19 codons have been inferred to be its optimal codons. The results show that pattern of the codon usage in P. pastoris is similar to that in S. cerevisiae and in K. lactis except for the synonymous codon of glutamic acid, which may be the special bias of P. pastoris.