CODON OPTIMIZATION AND RIBOSOME PROFILING FOR INCREASING TRANSGENE EXPRESSION IN CHLOROPLASTS OF HIGHER PLANTS

摘要

Methods for improving transgene in chloroplasts are disclosed along with improved transgenes so produced and methods of use thereof for the treatment of disease. Specifically, the methods comprising analyzing the native sequence of a nucleic acid encoding a protein of interest and replacing codons in said sequence with those preferentially used in psbA genes in chloroplasts in higher plants.