trxS基因对大麦发芽籽粒中蛋白质降解的影响

摘要

对转trxS基因大麦籽粒发芽过程中蛋白酶活性、不同蛋白组分含量和贮藏蛋白SDS-PAGE图谱的变化进行了研究.结果表明:与对照相比,转基因籽粒中的蛋白酶活性提高;清蛋白、球蛋白、醇溶蛋白和谷蛋白含量低于对照.SDS-PAGE图谱也表明,转基因籽粒中贮藏蛋白降解快于对照.%We assessed the effects of trxS gene on changes of proteinase activity,contents of different protein fractions and SDS-PAGE profiles in germinating seeds of contrasting transgenic and nontransgenic barley variety.Proteinase activity was enhanced by 70.28% in transgenic than nontransgenic barley seeds,whereas contents of albumin,globulin,hordein and glutelin in transgenic seeds were 3.68%,23.52%,31.37%,and 21.04%,lower than those in nontransgenic seeds.Degradation rates of hordein and glutelin in transgenic seeds were faster than those in nontransgenic seedlings as indicated by the SDS-PAGE profiles.Our data imply that the transformation of trxS gene could promote the degradation of protein, providing theoretic basis for the use of trxS gene and barley quality breeding.