乳酸锌和氟化亚锡对铜绿假单胞菌、鲍曼不动杆菌和变异链球菌生物被膜的抑制作用

摘要

乳酸锌(Zn lactate·3H2O)和氟化亚锡(SnF2)常作为牙膏中的活性物质添加剂用来预防龋齿及口腔生物被膜的形成.文中评估了Zn lactate.3H2O和SnF2对铜绿假单胞菌、鲍曼不动杆菌和变异链球菌生物被膜的作用.对铜绿假单胞菌PAO1生物被膜的抑制实验证实乳酸锌和氟化亚锡都具有抑制其生物被膜的功能,联用效果尤佳.乳酸锌通过干扰胞外多糖基质网的形成起作用,而氟化亚锡则可以明显降低生物被膜的生物量.更为重要的是,工作浓度的两种化合物联用几乎可以完全抑制3种实验菌株生物被膜的形成.%Zn lactate and SnF2 were used as active compounds in the dentifrice.Here,their anti-biofilm effects were evaluated on Pseudomonas aeruginosa,Acinetobacter baumannii and Streptococcus mutans.The biofilm prevention/dispersal assay ofP.aeruginosa PAO1 demonstrated that Zn lactate and SnF2 can inhibit biofilm formation independently or by combined treatment.Zn lactate disrupted extracellular polysaccharides matrix formation and SnF2 reduced the biomass of biofilm.Most importantly,the combination of Zn lactate and SnF2 thoroughly abolished the biofilm formation of all three strains.