表达猪圆环病毒2型Cap蛋白的重组猪瘟兔化弱毒疫苗株的构建与鉴定

摘要

Classical swine fever (CSF),caused by classical swine fever virus (CSFV),is a devastating viral disease in swine,leading to significant economic losses to the pig husbandry.C-strain is one of the best modified live vaccines against CSF.The vaccine is highly safe and efficacious and can provide rapid and complete protection against essentially all genotypes of CSFV.Co-infections of pigs with CSFV and porcine circovirus type 2 (PCV2) occur frequently in the field,making it difficult to control the associated diseases.Here,a recombinant C-strain rHCLV-Cap expressing the Cap protein of PCV2 was constructed and evaluated in vitro and in vivo.The recombinant had comparable phenotypes to C-strain in cell cultures and rabbits.At ten days post-immunization,anti-E2,but not anti-Cap,antibodies were detected in the rabbits inoculated with the recombinant virus.Our study warrants further work to construct C-strain-based bivalent vaccines.%猪瘟(CSF)是由猪瘟病毒(CSFV)引起的一种毁灭性传染病,给养猪业造成重大经济损失.猪瘟兔化弱毒疫苗(C株)是一株非常安全、有效的优秀弱毒疫苗,对各年龄和品种的猪都极其安全,同时对不同基因亚型的CSFV均能提供有效的免疫保护.在现地,CSFV和猪圆环病毒2型(PCV2)混合感染的现象时常发生,有必要研制针对这两种病毒混合感染的二价疫苗.本研究首次构建了表达PCV2 Cap蛋白的重组C株,并评价了其在体内外的特性.结果表明,该重组病毒与C株具有相近的体外增殖特征,能够稳定表达Cap蛋白,在家兔体内具有与C株相似的生物学表型,在免疫家兔后10d,抗CSFV E2抗体全部转阳,然而抗Cap抗体未能转阳.本研究为进一步优化表达PCV2Cap蛋白的重组C株奠定了基础.