The essential structure and the principle of continuous countercurrent extraction equipment on Chinese herbal medicine were introduced.The experiment on extraction effective components of Gynostemma pentaphyllum Makino was studied by water as solvent and G.pentaphyllum Makino as stuff.The key factors influencing extraction yield of wate-solubile matter and saponins, which including extracting temperature, ratio of liquid to solid and extracting time were investigated by using orthogonally experimental design.The results showed that the optimum condition were as follows: liquid to solid ratio 1: 35, temperature 80 ℃, time 50 min.Under these conditions, and the yield of extract was 33.95% and the yield of gypenosides was 8.9%.%简述了中草药连续逆流提取机的基本结构和工作原理.以水为溶媒、绞股蓝为原料,进行绞股蓝有效成分的提取试验.以绞股蓝水溶出物和总皂苷为目标物,考察提取温度、液料比和提取时间等因素对提取得率的影响,并通过L9(33)正交实验设计,对提取工艺进行优选.采用中草药连续逆流提取机提取绞股蓝有效成分的最佳工艺条件:提取溶剂温度80 ℃,料液比1:35(g/mL),提取时间50 min;所得提取物得率为33.95%,总皂苷得率为8.9%.
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