Based on the YG media, effects of different conditions to the growth and phosphorus accumulation rate of JPA1 strain were discussed. Result showed that the logarithmic phase of JPA1 was from 3 h to 9 h, it turned into stationary phase after 9 h, and then entered the state of death phase. Single factor experiment showed that maltose was the optimal carbon source for JPA1. The optimal growth temperature was 30 ℃ to 35 ℃. The best pH was 8. Metal ions of Na+ , Ca 2+ , Mg 2+ , Mn 2+ , and K+ were benefical to the growth of JPA1. The optimal phosphorus accumulation temperature was among 30 ℃ to 35 ℃ , optimal phosphorus accumulation pH was 7 , Metal ions of Fe2 + , Na + , Mg2 + , Mn2 + , and K + were good for phosphorus accumulation. Based on the YG media, using maltose to replace glucose, orthogonal experiment results showed that the best phosphorus accumulation condition was maltose 1. 5 g/L, 37 ℃ , pH 6. 5, volume of liquid 50 mL (shake flask culture in 300 mL) ; the best condition of culture was maltose 1. 5 g/L, 37 ℃ , pH 6. 5 or 7. 5, volume of 150 mL (shake flask culture in 300 mL).%以YG培养基为基础,考察不同条件对聚磷菌JPA1菌株生长及聚磷效率的影响.结果表明:JPA1菌株生长的对数期为3～9h,9h后进入稳定期,12 h到达衰亡期.在单因素试验中,麦芽糖为JPA1菌株最适生长和聚磷的C源,最适生长温度为30～ 35℃,pH为8,Na+、Ca2+、Mg2-、Mn2+和K+等离子有利于JPA1菌株的生长；最适聚磷温度为30 ～ 35℃,pH为7,Fe2+、Na+、Mg2+、Mn2+和K+等离子有利于JPA1菌株的聚磷.正交试验结果表明:JPA1菌株去磷培养基的最优组合为麦芽糖1.5 g/L,温度37℃,pH 6.5,装液量50 mL(300 mL摇瓶)；菌株最适扩增培养基为麦芽糖1.5 g/L,温度37℃,pH 6.5或7.5,装液量150 mL(300 mL摇瓶).
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