为探究不同材质砧板对卤猪舌中单增李斯特菌到黄瓜转移率的影响，选定木质、塑料和不锈钢为材质研究对象，根据消费者生活习惯设定砧板放置时间0、6和18 h，按照文献调研结果设定4种常见的场景，模拟家庭厨房食品制备过程，测定单增李斯特菌在卤猪舌、砧板和黄瓜之间的污染水平。同时，6和18 h后切黄瓜的试验中，通过结晶紫染色与显微镜观测，分析不同材质砧板上单增李斯特菌的生物菌膜形成情况。结果表明：4种场景下，砧板到黄瓜的转移率变化趋势相似，即同种材质砧板场景1、2、3和4呈现依次递减的趋势；不同材质砧板同种场景下，木质砧板到黄瓜的转移率较高，塑料和不锈钢砧板的转移率近似。此外，6和18 h单增李斯特菌膜并未形成，即短时间内散状的菌落个体无法繁殖成环状的菌膜结构。%The purpose of this work was to explore the influence of different cutting board materials on the transfer rate of Listeria monocytogenes from marinated pig tongue to cucumber.The wooden cutting board, one plastic cutting board and one stainless steel cutting board were used.These cutting boards were stayed about 0, 6 and 18 h according to the consumers′ habits. Meanwhile, transferring experiments were performed to mimic the food preparation process by setting four scenarios. And the contamination level ( lg( CFU/g) ) of marinated pig tongue,cutting boards and cucumber were also determined. At the same time,the biofilm formation on different material cutting boards was detected by crystal violet stain and microscope observation.The results showed that the scenario 1,2,3,4 descended orderly under the cutting board of same material,and the transfer rate of wood cutting board was significantly higher than that of the other two cutting boards under the same scenario.The transfer rate of L. monocytogenes from plastic cutting board to cucumber was similar to those from plastic cutting board to stainless steel cutting board. In addition,biofilm of L. monocytogenes was not because the strain could not form a cyclic structure within the treatment of 6 and 18 h.
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