大肠杆菌芳香族氨基酸代谢工程研究进展

摘要

Aromatic amino acids (AAAs),including L-phenylalanine (L-Phe),L-tyrosine (L-Tyr) and L-tryptophan (L-Trp),are important essential amino acids in the organism,and have important biological functions and wide applications in the fields of pharmaceutic,food and feed industries so on.This paper introduces biosynthetic pathway and metabolic regulation of AAAs, and systematically summarizes the metabolic engineering strategies for the construction of Escherichia coli AAAs producing strains.In view of the problems in industrial production of AAAs at present,this paper prospects the further application of metabolic engineering strategies for the modification of AAAs producing strains.%芳香族氨基酸包括L苯丙氨酸(L Phe)、L酪氨酸(L Tyr)和L色氨酸(L Trp),是生物体内非常重要的必需氨基酸,具有重要的生物学功能,广泛应用于医药、食品和饲料等领域.本文中,笔者介绍了芳香族氨基酸的生物合成途径以及代谢调控,综述了构建大肠杆菌芳香族氨基酸生产菌株的代谢工程策略.针对现阶段工业化生产芳香族氨基酸存在的问题,笔者对进一步应用代谢工程策略改造芳香族氨基酸菌株进行了展望.