黑曲霉果胶裂解酶的生物信息分析

摘要

用生物信息方法对果胶裂解酶(PNL)基因的核酸序列及其推导氨基酸序列的组成、亚细胞定位、疏水性/亲水性以及二、三级结构等进行分析.结果表明,黑曲霉的PNL为具有一定亲水性的稳定酸性分泌蛋白,具有明显的信号肤,无跨膜结构区,保守功能结构域为Pee_lyase_C.二级结构主要构成是不规则卷曲,具有以β片层结构为基础的相似三维空间结构.%Analyzed and predicted the nucleotide and amino acids composition、sub -cell location,signal peptides and tran -membrane regions、hydrophobicity/hydrophilicity ..secondary and tertiary structure of pectin lyases including Aspergillus niger from NCBI database. Results shows that A. Niger PNLs are stability of hydrophilic secreted protein, with a clear signal peptide, non - transmembrane area. The structures of PNL include a characteristic functional domain of Pec_lyase_C. Random coil is the main components of its secondary structure and has similar tertiary structures with the β sheet structure.