应用电子克隆技术,获得甘蔗中一个过氧化氢酶基因的cDNA序列全长,命名为S- CAT.该基因全长2 160 bp,包含一个1 479 bp的开放阅读框,编码492个氨基酸.通过PSORT工具,预测甘蔗S- CAT蛋白存在于过氧化物酶体中.同源比对分析显示,S - CAT基因编码的氨基酸序列与水稻、玉米、高梁、朝鲜碱茅、葡萄等植物中过氧化氢酶基因所编码的氨基酸序列高度同源,同源性分别为97％,97％,95％,91％和92％.研究结果为该基因的实验克隆奠定基础.%A catalase gene from Saccharum officinarum, termed as S - CAT, was cloned in silico based on the corresponding EST sequences from sugarcane EST database in NCBI. Several characters of the amino acid encoded by S - CAT gene were analyzed and predicted by bioinformatics tools, including the composition of amino acid sequence, hydrophobicity and hydrophilicity, secondary structure of protein and so on. Bioinformatics analysis also revealed that the full - length of S - CAT gene from S. Officinarum was 2 160 bp, containing a complete ORF encoding 492.amino acids. Using PSORT, it was predicted that the S - CAT protein was located in peroxisome and belonged to the CAT - POD type of catalases. Homology comparison and phylogenetic analysis showed that the amino acid encoded by S - CAT gene in sugarcane was highly homologous with those encoded by CAT gene in rice, maize, sorghum, Korean alkaligrass and grape, with homologies of 97 % , 97 % 95 % , 91 % and 92 % , respectively. The above results will provide tHe basis for molecular cloning of CAT genes in;sugarcane.
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