微波加热破膜提取猪血超氧化物歧化酶的研究

摘要

目的建立一种提取猪血超氧化物歧化酶粗品的简易方法。方法采用微波加热和铜离子保护相结合的方法从猪血红细胞中提取超氧化物歧化酶。结果微波加热破膜法与传统的水溶胀法相比，处理时间短，酶的比活显著提高。结论微波加热破膜法是一种快速、高效的粗提取超氧化物歧化酶的方法。%Purpose　The aim is to establish a simple method to extract superoxide dismutase(SOD) from pig blood red cells.Methods　A combined method-microwave heating and adding Cu2+ into the suspension-was used to extract SOD from the red cells. Results　The undesired proteins were denatured by microwave heating and SOD was partly purificated in comparison with hemolysis. The processing time of the former was much shorter than the latter. Conclusion　A novel cell disruption microwave heating, was a rapid and effective technique for the primary extraction of SOD.