莲心碱对氯化锂-匹鲁卡品癫痫模型大鼠皮层脑电图的影响

摘要

目的：本实验旨在探讨莲心碱对氯化锂-匹鲁卡品致癫大鼠皮层脑电图的影响，进一步研究莲心碱的抗癫痫作用谱。方法32只雄性SD大鼠随机分为4组：低剂量莲心碱组（2.5 mg/mL）、高剂量莲心碱组（5 mg/mL）、生理盐水组作为阴性对照组、左乙拉西坦组（100 mg/mL）作为阳性对照组，每组8只。建立氯化锂-匹鲁卡品癫痫模型。行为学确定建模成功后，将大鼠麻醉固定于立体定位仪，根据George Paxions图谱将套管针插入左侧侧脑室，留置套管针备用。安置皮层脑电图记录电极。记录脑电图30 min后，各组大鼠分别用微量注射器沿留置管缓慢注射不同浓度的莲心碱、左乙拉西坦和生理盐水各10μL，之后继续记录脑电图150 min。分别观测给药前30 min与给药后每30 min癫痫样放电次数的变化。记录同组内单位时间癫痫样放电次数，并进行前后比较，以及比较同时段癫痫样放电次数变化。结果低剂量莲心碱组、高剂量莲心碱组和左乙拉西坦组注射后单位时间癫痫样放电次数呈下降趋势，其中低剂量莲心碱组注射60 min后下降具有显著统计学差异（P＜0.01）、高剂量莲心碱组30 min后下降有显著性差异（P＜0.01）；左乙拉西坦组注射30 min内即有明显降低（P＜0.01）；生理盐水组注射后单位时间癫痫样放电次数变化无统计学差异。莲心碱组单位时间癫痫样放电次数与同时段左乙拉西坦组进行比较，高剂量莲心碱组注射后各时段癫痫样放电次数变化与左乙拉西坦组比较均无统计学差异；低剂量莲心碱组注射后30～60 min时间段癫痫样放电次数没有左乙拉西坦组减少明显（P＜0.05），其余各时段2组癫痫样放电次数变化无统计学差异。结论莲心碱对氯化锂-匹鲁卡品癫痫模型大鼠急性期皮层脑电图癫痫样放电具有抑制作用。%Objective To explore the effect of liensinine on cortical EEG of epileptic rats induced by lithium chloride-pilocarpine,and investigate the effective spectrum of liensinine on epilepsy.Methods 32 SD rats were randomly divided into four groups:low dose of liensinine group(2.5 mg/mL, 10μL),high dose of liensinine group(5 mg/mL,10μL),the normal saline group(10μL)which was negative control group,levetiracetam group (100 mg/mL,10μL)which was positive control group,8 rats in each group.Electrocorticogram of rats was recorded after chloride lithium-pilocarpine model was induced.The anesthetic rats were fixed on stereotaxic apparatus after the epilepsy model was confirmed by ethology.A trochar was put into the left lateral ventricle.Rats were implanted with epidural recording electrodes.After the cortical EEG was recorded about 30 minutes, liensinine (at concentration of 2.5,5 mg/mL),levetiracetam and 0.9% sodium chloride was injected into lateral ventricle.Electrocorticogram was recorded about 150 minutes again.The frequency of epileptic discharge was observed every 30 minutes.The differences of frequency in the same group and the different change of frequency between groups at the same period were compared.Results The frequency of epileptic discharge decreased in low dose of liensinine group,high dose of liensinine group and levetiracetam group after administration ,there was significantly statistical difference in low dose of liensinine group after administration about 60 minutes(P<0.01 ),there was significant statistics difference in high dose of liensinine group after administration about 30 minutes(P<0.01),and the same change in levetiracetam group within 30 minutes after administration(P<0.01);the change of frequency of epileptic discharge was no significantly statistical difference between pro-and post-administration in the normal saline control group.The difference of the frequency change in epileptic discharge at the same period between liensinine group and levetiracetam group was observed ,there was statistic difference between low dose of liensinine group and levetiracetam group at the period of thirty to sixty minutes after administration,there was no statistic difference at other periods;there was no statistic difference between high dose of liensinine group and levetiracetam group at every period.Conclusion Liensinine could inhibit the epileptic discharges in acute model of epileptic rats induced by chloride lithium-pilocarpine.