科罗索酸的抗肿瘤作用及其对CAM和YS M血管生成的影响

摘要

Objective To evaluate the antitumor effect of corosolic acid and its impact on CAM and YSM angiogenesis.Methods The effects of CRA on A549 proliferation was studied by MTT method in vitro.Tumor-bruden nude mice model were established by injecting A549 lung cancer cell marked with bioluminescent to nude mice,and the growth of tumor in mice were detected by IVIS small animal in vivo imaging system.Experiment of chicken embryos eggs are used to observe the role of drugs on CAMand YSMblood vessels. Results The value of IC50 of CRA for A549 in vitro was 26.8μg/mL.A tumor-burdened animal experiment results showed that the CRA to A549 solid tumor has a certain therapeutic effect.CAM and YSM blood vessels of chicken embryos eggs treated with CRA were decreased significantly than negative control group.Conclusion CRA has certain therapeutic effect for A549,which may be related to the inhibition of angiogenesis in tumor tissues.%目的：探究科罗索酸（corosolic acid，CRA）的抗肿瘤作用及其对鸡胚绒毛尿囊膜（chick chorioallantoic membrane，CAM）和卵黄囊膜（yolk sac membrane，YSM）血管生成的影响。方法 MTT法检测CRA对肺癌细胞A549增殖的影响。将生物发光标记的人肺癌细胞A549接种到裸小鼠体内，建立生物发光标记的裸小鼠荷瘤模型，采用IVIS小动物活体成像系统检测小鼠体内肿瘤生长情况。采用鸡胚孵化实验，观察CRA对CAM和YSM血管生成的影响。结果 CRA对A549增殖抑制的IC50值为26.8μg/mL。荷瘤动物试验结果表明：CRA对A549实体瘤有显著的抑制生长作用（P＜0.01）。在鸡胚孵化实验中，CRA可明显降低CAM和YSM血管生成。结论 CRA对A549具有一定的治疗作用，其作用机制可能与抑制肿瘤组织中血管生成有关。