中药复方益糖康对DN模型大鼠肾脏组织中AGEs、RAGEs影响的实验研究

摘要

Objective To observe the effect of Chinese herbal compound Yitangkang on the expressions of advanced glyclation end-products (AGEs) and the receptor of advanced glyclation end-products mRNA(RAGE-mRNA) in rats with diabetic nephropathy(DN),and to discuss the mechanism of the formulas in the treatment of DN. Methods The rat model of DN were established by intraperitoneal injection of strepotozotocin(STZ). After 8 weeks intervention with medications, the expression of renal RAGEs mRNA was detected by Real-time PCR, renal RAGE by immunohistochemistry, and renal content of AGEs by spectrophotometry. Results Compared with normal control group, the expression of renal RAGEs mRNA and RAGEs in diabetic nephropathy rats were increased significantly(P<0.01). Compared with DN group, the expression of renal RAGEs mRNA and RAGEs in three doses Yitangkang groups and western medicine group were decreased significantly(P<0.01). Conclusion Chinese herbal compound Yitangkang could protect kidney of diabetic nephropathy rats,and the mechanism is related to its loweirng renal expression of RAGEs mRNA, RAGEs and AGEs content.%目的：观察中药复方益糖康对糖尿病肾病大鼠肾皮质中晚期糖基化终末产物（advanced glyclation end-products，AGEs）及其受体的影响，探讨其对糖尿病肾病的防治作用机制。方法采用链脲佐菌素（strepotozotocin，STZ）诱导大鼠糖尿病模型，观察8周时肾脏组织AGEs、RAGE mRNA及蛋白表达的变化，以及经中药复方益糖康干预后的变化。结果糖尿病大鼠8周时，模型大鼠肾脏组织中的AGEs含量显著高于空白组（P<0.01），而中药预防组、中药低剂量组、中药高剂量组、西药组大鼠肾脏组织中AGEs含量较模型组显著下降（P<0.01）；糖尿病肾病模型组大鼠肾脏组织中糖基化终末产物受体（receptor of advanced glyclation end-products，RAGE）mRNA表达水平较空白组显著升高（P<0.01），而中药预防组、中药低剂量组、中药高剂量组、西药组大鼠肾脏组织中RAGE mRNA表达水平较模型组显著下调（P<0.01）。结论中药复方益糖康对糖尿病大鼠肾功能的保护作用，与其抑制AGEs/RAGE的激活有关。