目的：评价多粘菌素B与美罗培南体外联合应用对临床分离的30株泛耐药铜绿假单胞菌的抗菌效应。方法采用微量肉汤稀释法和棋盘法测定多粘菌素B与美罗培南单独用药和联合用药后，临床分离的30株泛耐药的铜绿假单胞菌的最小抑菌浓度（minimal inhibitory concentration，MIC），及计算部分抑菌浓度指数（fractional inhibitory concentration，FIC），根据FIC指数判定联合效应类型，判断是否有协同或拮抗作用。结果多粘菌素B与美罗培南联用后，药物对泛耐药铜绿假单胞菌的MIC值均显著降低，FIC指数在0～0.5百分率为60%；FIC指数在0.5～1.0的百分率为30%。结论多粘菌素B与美罗培南联合用药后，以协同作用和相加作用为主，测定出分离的30株泛耐药铜绿假单胞菌的抗菌作用无拮抗作用。%Objective To evaluate the anti-bacteria effects of polymyxin B combined with meropenem against 30 strains pan-drug resistant pseudomonas aeruginosa that separated in clinic. Methods The minimal inhibitory concentration (MICs) of 30 strains pan-drug resistant pseudomonas aeruginosa after treated with polymyxin B and meropenem as single-use or combination use were determined by both microdilution method and checkerboard method. The FIC index was calculated, then the type of combination effect was determined according to FIC index, which was used to determine whether there was synergistic or antagonistic effects. Results The MICs of pan-drug resistant pseudomonas aeruginosa were reduced significantly after polymyxin B combined with meropenem when compared with single-use. The percentages of the FIC index that less than 0.5 and the index between 0.5 and 1 were 60%and 30%respectively. Conclusion The results indicate that the combinations of polymyxin B with meropenem have good synergistic and additive effects against pan-drug resistant pseudomonas aeruginosa, there is no antagonism.
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