香菇多糖对顺铂抑制Hela宫颈癌细胞增殖作用的影响

摘要

目的：研究香菇多糖对顺铂抑制Hela宫颈癌细胞增殖的影响。方法根据处理方法将Hela宫颈癌细胞分为LEN组（单用香菇多糖处理）、CIS组（单用顺铂处理）、LEC组（香菇多糖联合顺铂处理）和CON组（未做香菇多糖和顺铂处理），比较其处理前后细胞增殖、细胞周期、PI和凋亡率的差异。结果4组Hela细胞处理前吸光度无明显差异，LEN组和CON组Hela宫颈癌细胞处理后d 1～d 8的吸光度无明显差异，均显著高于CIS组和LEC组Hela细胞（P<0.05）；CIS组Hela细胞处理后d 1～d 8的吸光度显著高于LEC组（P<0.05）。处理后CON组和LEN组两组Hela细胞G 0/G1期和凋亡率均显著低于CIS组和LEC组（P<0.05），S期和G 2/M期显著高于CIS组和LEC组（P<0.05）；LEC组Hela细胞G 0/G1期和凋亡率均显著高于CIS组（P<0.05），S期和G 2/M期显著低于CIS组（P<0.05）。结论香菇多糖可显著增强顺铂对Hela细胞增殖的抑制作用。%Objective To study the effect of lentinan on cisplatin’s inhibition on cervical cancer Hela cell line. Method Cervical cancer Hela cell line were divided into LEN group (only lentinan treatment), CIS group (only Cisplatin treatment), LEC group (Both Lentinan and Cisplatin treatment) and CON group (no Lentinan and Cisplatin treatment) according to their treatment methods. The differences of cell proliferation, cell cycle, PI and apoptosis were compared. Results The differences of absorbance between LEN group and CON group were not significant d1-d8 after treatment, but significant between CIS and LEC group (P<0.05). The absorbance in LEC group was significantly lower than in CIS group (P<0.05). The differences of PI and apoptosis rate in LEC and CIS group were significant (P<0.05), but not between LEN and group. The PI in LEC group was lower than in CIS group (P<0.05), while apoptosis rate was higher (P<0.05). Conclusion Lentinan could significantly promote Cisplatin’s inhibition effects on cervical cancer Hela cell line.