骨质疏松症脾肾两虚型病证结合动物模型的建立

摘要

目的:建立骨质疏松症脾肾两虚型动物模型,为中医药治疗骨质疏松症的研究提供工具.方法:采用雌性大鼠卵巢切除法建立肾虚型骨质疏松模型,在此基础上灌胃给予200％大黄浓煎液以建立脾肾两虚型骨质疏松模型,并采用骨组织形态计量学方法检测大鼠胫骨的骨量、骨形成及骨吸收情况以对建立的模型进行验证.结果:脾虚模型组和脾肾两虚模型组大鼠均毛色不光、食量减少、体重下降、行为迟缓、喜聚堆、弓背蜷缩、稀便等与临床脾虚证相似的证候特征.与假手术组相比,肾虚及脾肾两虚模型组大鼠胫骨TBV％显著降低,TRS％、TFS％、OSW、MAR和mAR显著增高,而脾虚模型组则无明显差异.与肾虚模型组相比,脾肾两虚模型组TBV％的降低,TRS％和TFS％的升高更为明显.结论:去卵巢能够造成大鼠肾虚型骨质疏松症,而单纯给予大黄浓煎剂可造成大鼠脾虚但不能导致骨质疏松症.若将去卵巢和灌胃给予大黄2种方法合用,可造成大鼠脾肾两虚型骨质疏松症,且发病程度较单纯去卵巢明显加重.该模型有明显的脾虚证候特征,且其骨质疏松的病理过程与人类绝经后骨质疏松症相似,能反映脾肾两虚型骨质疏松患者的发病特点,具有可行性.%Objective Establish a rat osteoporosis model with spleen and kidney deficiency syndrome to provide tools for the Chinese medicine study of the osteoporosis treatment. Methods Establish a rat osteoporosis model with kidney deficiency syndrome by Ovariectomy, On this basis, administrate of 200% rhubarb concentrated decoction orally to establish a rat osteoporosis model with spleen and kidney deficiency syndrome. And Validate the model by measuring the bone mass, bone formation and bone resorption of rat tibial. Results The symptom of rats of spleen deficiency group and spleen and kidney deficiency group were similar to osteoporosis patients with spleen deficiency syndrome, such as appeared sparse dry dull coat, eating less, weight loss, lazy move, get together, bowed back,loose stools. Compared with the sham operation group, TBV% in kidney deficiency model group and spleen and kidney deficiency model group decreased significantly, TRS% s TFS% 、OSW、MAR and mAR increased significantly, while Spleen model group was not significantly different. Compared with the kidney deficiency model group, TBV% in spleen and kidney deficiency model group decreased obviously, TRS% and TFS% increased significantly. Conclusion Ovariectomized rat kidney can cause osteoporosis with kidney deficiency syndrome, Simply give the rhubarb concentrated decoction can cause rat spleen deficiency syndrome, but can not lead to osteoporosis. If the two methods combined, which can lead to rat Osteoporosis with spleen and kidney deficiency syndrome, and the disease severity is more serious. The model has obvious spleen deficiency syndrome characteristics, and the pathological process of osteoporosis similar with human postmenopausal osteoporosis. It can reflect the clinical characteristics of the spleen and kidney deficiency in patients with osteoporosis, so it is feasible.