松郁安神方对REM睡眠剥夺大鼠认知功能及神经递质的影响

摘要

Objective: To observe the effects of the compound Song-Yu-An-Shen-Fang on learning and memory capability and levels of monamine-neurotransmitter in REM sleep deprivation rats. Methods: The rats were randomly divided into normal group, environment control group, REM sleep deprivation group, Song-Yu-An-Shen-Fang treatment group and alprazolam treatment group. Morris water maze was used to measure Learning and memory performance. The levels of NE, DA and 5-HT were determined by HPLC-ECD. Results; Song-Yu-An-Shen-Fang improved the learning and memory capability of REM sleep deprivation rats and regulated the levels of NE, DA and 5-HT in plasma. Conclusion: Song-Yu-An-Shen-Fang can significantly improve the learning and memory capability in REM sleep deprivation rats, the underlying mechanism may be related to regulate the levels of some neurotransmitters.%目的:研究松郁安神方对REM睡眠剥夺大鼠认知功能及血浆中神经递质的影响.方法:药物治疗5d后水迷宫实验观察大鼠的学习记忆,用高效液相-电化学检测NE、DA、5-HT的含量.结果:中药治疗后大鼠的学习记忆能力较模型组明显改善,NE含量明显降低,DA、5-HT含量明显升高.结论:松郁安神方不仅可改善REM睡眠剥夺大鼠的学习记忆能力,而且可调节大鼠睡眠相关物质NE、DA、5-HT的含量,其学习记忆能力的改变可能与某些神经递质的改变有关.