Objective:To observe the effect on blood lipids of Yueju pills,a representative formula of the SixYu,thus provide reference for clinical application and experimental study.Methods:The model of atherosclerotic plaque was established by feeding ApoE-/-mice with high-fat diet.Interventions include Yueju pills and simvastatin.Elisa was used to detect the serum lipid content in mice.The intestinal microflora was analyzed based on 16S rDNA gene sequencing.Results:Mice in Yueju pills group,simvastatin group as well as the model control group experienced higher level of serum cholesterol (TC),triglyceride (TG),low density lipoprotein (LDL-C) and lower level of high-density lipoprotein (HDLC) than those in the normal diet control group.TC and LDL-C levels of mice in Yueju pills group were lower than those in model control group but showed no significant difference compared with simvastatin group.In terms of triglyceride (TG)and high-density lipoprotein (HDL-C),Yueju pills group showed no statistically difference compared with model control group.There was a significant difference in the stool samples between Yueju pills group and the other groups,while the difference between the model control group and the simvastatin group was not significant.Conclusion:Yueju pills can reduce TC and LDL-C,whose mechanism is different from simvastatin.Yueju pills may reduce TC and LDL-C by inhibiting the growth of four kinds of bacteria such as Coriobacteriaceae,while promoting the growth of Bifidobacteriaceae and promoting growth of five other kinds of bacteria such as bifidobacterium.%目的:观察治疗六郁的代表方越鞠丸对血脂的影响,为临床应用和实验研究提供参考依据.方法:采用高脂饲料饲养A poE-/-小鼠的方法建立动脉粥样硬化斑块模型,用越鞠丸和辛伐他汀分别对高脂饲料饲养的ApoE-/-小鼠进行干预,ELISA法检测小鼠血清血脂含量,基于16S rDNA基因测序技术分析小鼠肠道菌群.结果:越鞠丸组、辛伐他汀组和模型对照组小鼠血清胆固醇(TC)、甘油三酯(TC)和低密度脂蛋白(LDL-C)含量明显高于正常对照组,高密度脂蛋白(HDL-C)含量明显低于正常对照组;越鞠丸组小鼠TC和LDL-C含量明显低于模型对照组且与辛伐他汀组比较差异无统计学意义,TG和HDL-C含量与模型对照组比较差异无统计学意义.越鞠丸组与正常对照组、模型对照组、辛伐他汀组小鼠粪便样本菌群存在显著性差异,模型对照组与辛伐他汀组小鼠粪便样本菌群比较差异无统计学意义.结论:越鞠丸能降低TC和LDL-C,其机制与辛伐他汀不同,越鞠丸可能通过抑制红蝽菌科等4类细菌和促进双歧杆菌科等5类细菌的生长来降低TC和LDL-C.
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