左金方体内外抑制幽门螺杆菌的实验研究

摘要

Objective:To study the antimicrobial activities of ZuoJinFang against helicobacter pylori in vivo and in vitro.Methods:The minimum inhibiting concentration (MIC) of ZuoJinFang and Clarthromycin were measured by agar dilution method;sixty BALB/c mice,half male and half female,were randomly divided into 6 groups,normal control group,model group,gastric triad group (0.351 mg· g-1) and ZuoJinFang low,middle and high dose groups (0.364,0.728,1.456 g· kg-1 · d-1),the mice were inoculated orally with antibiotic on first day and with Helicobacter pylori strains for 7 continuous days to establish Hp infection model.From experimental day 11,the mice were given oral administration of the corresponding drugs for 8 weeks.At the end of the experiment,gastric mucosa and serum were obtained for serum HP antibody test and pathological section examination.Results The MIC of ZuoJinFang was confirmed as 200 μg· mL-1 and that of Clarthromycin was 0.5 μg· mL-1.The result of serum antibody test and pathological section examination showed that Hp infection model had been established successfully,compared with the normal control,were statistically significant.And both gastric triad group and ZuoJinFang could inhibit the growth of Hp in vivo,compared with the model group,were statistically significant.Conclusion:ZuoJinFang are effective on inhibiting the growth of Hp in vivo and in vitro.%目的:探讨左金方在体内外抑制幽门螺杆菌(hlicobacter pylori,Hp)生长繁殖的作用.方法:采用琼脂稀释法测定左金方和克拉霉素对Hp最小抑菌浓度(minimum inhibitory concentration,MIC).60只BALB/c小鼠雌雄各半,按体质量分层随机分为正常组、模型组、胃三联组(0.351 mg·g 1)及左金方低、中、高剂量组(0.364,0.728,1.456 g·kg-1·d-1)6组,采用抗生素加Hp的方法连续灌胃7d建立Hp感染动物模型.从第11天开始连续灌胃给药8周后处死小鼠,进行血清尿素酶抗体检测以及病理切片检查.结果:琼脂稀释法测得左金方最小MIC值为200 μg· mL-1,克拉霉素最小MIC值为0.5 μg· mL-1.血清幽门螺杆菌抗体检测、小鼠胃黏膜病理切片结果均提示Hp感染动物模型复制成功,与正常对照组比较差异有统计学意义.胃三联组、左金方高、中、低剂量组均能有效抑制体内Hp的生长,与模型对照组比较差异有统计学意义.结论:左金方在体内体外均能有效地抑制幽门螺杆菌的生长.