荷叶水提物对实验性肥胖大鼠脂代谢的影响及机制

摘要

Objective:To investigate the lipid metabolic effect and mechanism of water extract of lotus leaves(Traditional Chinese Medicine).Methods:Isolated SD rat lipid tissues were suspended in organ baths containing Krebs solution,and the effect of lotus leaf water extract on free fatty acids (FFA) release was observed;The experimental obesity rat model was established by feeding them high glucose and fat diets,then the changes of body weight and blood lipid were measured in the model rats after intragastric administration with water extract of lotus leaves for four weeks,and the expressions of peroxisome proliferator-activated receptor gamma (PPAR-γ) and leptin were examined by RT-PCR and immunohistochemical.Results:The ex vivo experiment showed that water extract of lotus leaves effectively promoted the FFA release from isolated lipid tissues.In vivo experiment,similarly to Orlistat,water extract of lotus leaves(60 mg/kg) markedly decreased the body weight and blood lipid of experimental obesity rats(P ＜ 0.05),and obviously reduced the expressions of PPAR-γ and leptin(P ＜ 0.05).Conclusion:Water extract of lotus leaves greatly improves the expression of PPAR-γ and leptin,which can promote the lipid mobilization and dissolution,reduce the body weight and blood lipid of adult rats with experimental obesity,therefore is expected to be developed into lipid-lowering diet pills.%目的:探讨荷叶水提物的减肥降脂作用及机制.方法:通过离体脂肪组织灌流实验观察荷叶水提物对正常SD大鼠离体脂肪组织游离脂肪酸(FFA)释放的影响;利用高糖高脂饮食诱导建立实验性肥胖大鼠模型,探讨荷叶水提物给药4周后,大鼠体重和血脂水平的变化,并采用RT-PCR和免疫组化技术对脂肪组织过氧化物酶体增殖物激活受体γ(PPAR-γ)和瘦素(leptin)表达进行检测.结果:离体实验发现荷叶水提物可明显促进离体脂肪组织FFA的释放.在体实验发现中剂量荷叶(60 mg/kg)与奥利斯他相似,可使实验性肥胖大鼠体重、血脂水平明显下降(P＜0.05),使脂肪组织PPAR-γ和瘦素的表达明显下降(P＜0.05).结论:荷叶水提物可通过改善大鼠脂肪组织PPAR-γ和leptin的表达,促进脂肪的动员和分解,降低肥胖大鼠体重和血脂水平,有望研发为减肥药物.