Objective To observe the expressions of nuclear factor - kB( NF-kB) and inducible nitric oxide synthase( iNOS) protein in the brains of premature rats to moderate hypoxia - ischemia ( HI) , in order to explore possible relationships with white matter damage ( WMD). Methods The 2 days old rats were randomly divided into experiment group and sham operation group. The pups were perfused at 1 h,3 h,6 h,12 h,l d,3 d and 7 d of recovery from HI. Immunohistochemical technical was applied to investigate the changes of expressions of NF-kB and iNOS in periventricular rats white matter tissues. Results In the experiment group,the expression of NF-kB in periventricular rats white matter started to increase at 1 h and reached the peak at 12 h[ (45.2 ±2.5) vs (35.7 ±2. 1) ; (62. 3 ±2. 9)vs (34. 1 ±2.0); (75.0 ±3. 7)vs (33.6 ±2.1) ;(126.2 ±4.3)vs (14.3 ±2.4);(81.1 ±2.6)vs (33.7 ±2.0) ;(40.2 ±2.5)vs (32.5 ±2.3); (34.6 ± 2. 6)vs (31.9 ±2.8),P. <0.001]. The expression of iNOS was up-regulated at 1 h and peaked at 1 d after HI [ (25.4 ±1.7) vs (22.7 ± 1.9);(37.9±1.9)vs (21.7±1.2);(53. 3 ±2. L)vs (21.9 ± 1.0); (61.9 ± 1.7)vs (21. 7 ± 1. 3); (74. 0 ±2. 2)vs (22.7 ±1.5); (57.3 ±2.2)vs (21.1 ±l.l);(26.3±2.S)vs (20.8 ±1.6) ,Pa <0.001]. The correlation analysis between the expressions of NF-kB and iNOS illustrated that they were correlated(r =0.977,P < 0.001). Conclusions HI can induce the prominent increase in the expressions of NF-kB and iNOS in white matter of the neonatal rats' brain,and may be involved in the pathophysiological process in the WMD.%目的 研究缺氧缺血(HI)对新生大鼠脑白质胶质细胞核转录因子κB(NF-κB)及诱导型一氧化氮合酶(iNOS)表达的影响,探讨NF-κB及iNOS在新生大鼠脑白质损伤(WMD)发病机制中的作用.方法 将2日龄大鼠随机分为实验组和假手术组,建立新生大鼠WMD模型,分别于HI后1h、3h、6h、12h、1d、3d及7d处死,对其脑组织取材后行HE染色观察其病理改变,并应用免疫组织化学法检测其NF-κB及iNOS的表达水平.结果 在新生大鼠脑白质中,实验组NF-κB表达水平于HI后1h开始明显上升,12h达到高峰,至7d仍明显升高,与对照组比较差异均有统计学意义[(45.2±2.5)vs(35.7±2.1)；(62.3±2.9)vs(34.1±2.0)；(75.0±3.7)vs(33.6±2.1)；(126.2±4.3)vs(14.3±2.4);(81.1±2.6)vs(33.7±2.0);(40.2±2.5)vs(32.5±2.3);(34.6±2.6)vs(31.9±2.8),Pa＜0.001].实验组iNOS于HI后1h表达开始显著增多,1d达到高峰,至7d仍明显升高,与对照组比较差异均有统计学意义[(25.4±1.7)vs(22.7±1.9)；(37.9±1.9)vs(21.7±1.2)；(53.3±2.1)vs(21.9±1.0)；(61.9±1.7)vs(21.7±1.3)；(74.0±2.2)vs(22.7±1.5)；(57.3±2.2)vs(21.1±1.1)；(26.3±2.5)vs(20.8±1.6),Pa＜0.001].实验组脑室周围白质区NF-κB表达与iNOS呈正相关(r=0.977,P＜0.001).结论 HI可导致新生大鼠脑白质NF-KB表达及iNOS水平显著增高,参与新生大鼠WMD的病理生理过程.
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