白刺链霉菌15-4-2中的抗MRSA活性成分研究

摘要

Objective To study the antibacterial constituents from the fermentation broth of Streptomyces albospinus 15-4-2. Methods Bioassay-guided column chromatography was used to separate the antibacterial components from the fermentation broth of Streptomyces albospinus 15-4-2. Results Seven compounds were isolated and identified as N-(2-hydroxy-1 -(4-methoxyphenyl)ethyl)acetamide (1), 2-(4-methoxyphenyl)-2-(propylamino) ethanol (2), cytoxazone (3), P-hydroxybenzoic acid (4), (2S,3R)-3-hydroxy-2-methylbutanoic acid (5), p-methylphenol (6) and p-dimethoxy benzene (7). Conclusion The antibacterial assay exhibited that compounds 1, 3, 4, 5 and 6 showed inhibitory effects on methicillin-resistant Staphylococcus aureus (MRSA), and it was the first time to report the anti-MRSA effects of 1, 3, 4 and 5.%目的 研究白刺链霉菌(Streptomyces albospinus) 15-4-2发酵液中的抗耐甲氧西林金葡菌(MRSA)活性成分.方法 通过活性跟踪,采用柱层析等方法对白刺链霉菌15-4-2的次生代谢产物进行分离纯化.采用光谱分析对活性成分进行结构解析.结果 分离鉴定了7个化合物,其结构分别为N-(2-羟基-1-(4-甲氧基苯基)乙基)乙酰胺(1)、2-(4-甲氧基苯基)-2-(丙胺基)乙醇(2)、cytoxazone(3)、对羟基苯甲酸(4)、(2S,3R)-3-羟基-2-甲基丁酸(5)、对甲基苯酚(6)和1,4二甲氧基苯(7).结论 抗菌活性测试结果表明化合物1、3、4、5和6具有抗耐甲氧西林金黄色葡萄球菌(MRSA)活性.其中,1、3、4和5的抗耐甲氧西林金黄色葡萄球菌活性为首次报道.