响应面法优化紫色链霉菌发酵液的发酵工艺及其抑菌活性研究

摘要

Objective The abuse of antibiotics has brought many problems,and isolating and identifying new Streptomyces producing secondary metabolites with antibacterial activity is a main method to develop new antimicrobial agents.Methods The tilter paper method was used to study the antibacterial activity of the Streptomyces violaceus fermentation broth and the response surface methodology was employed to optimize the fermentation process of liquid fermentation.Results The Streptomyces violaceus fermentation broth had the highest antibacterial activity against Staphylococcus aureus;high antibacterial activity against Staphylococcus albus and Pastevula mulfocida,weak antibacterial activity against Salmonella typhimurium,Escherichia coli and Rhizopus chinensis,and no antibacterial activity against Bacillus subtilis,Pseudomonas aeruginosa and Aspergillus niger.The optimum fermentation process was obtained as follows:glucose + soluble 20.23g/L,peptone 2.54g/L,NaCl 0.76g/L,and K2HPO4 0.99g/L.Conclusion Under the optimal conditions,the inhibitory zone diameter of Streptomyces violaceus fermentation broth was 19.63mm.The results of the antibacterial activity showed that the Streptomyces violaceus fermentation broth had strong inhibitory effects against bacteria,but weak inhibitory effects against fungi.%目的 抗生素的滥用带来了很大的问题,分离并寻找新的具有抑菌活性的次生代谢产物的链霉菌是开发新型抗菌药物的主要手段.方法 采用滤纸片法研究紫色链霉菌发酵液的抑菌活性,并通过响应面法优化其液体发酵产抑菌活性物质的发酵工艺.结果 紫色链霉菌发酵液对细菌有较强的抑菌作用,而对真菌的抑菌作用较弱、对金黄色葡萄球菌的抑菌作用最强,对白色葡萄球菌和禽巴杆菌有较强的抑菌作用,对沙门菌、大肠埃希菌和中华根霉有较弱的抑菌作用,对枯草芽孢杆菌、铜绿假单胞菌和黑曲霉没有抑菌效果.经响应面法优化后,紫色链霉菌液体发酵的最优工艺参数为葡萄糖+可溶性淀粉20.23g/L、蛋白胨2.54g/L、NaC1 0.76g/L、K2HPO4 0.99g/L.结论 在此条件下,紫色链霉菌发酵液的抑菌圈直径为19.63mm,比优化前提高了18.9％.本研究为筛选新的天然抗菌活性物质提供了一定的理论基础.