The aim of this study was to use genome-wide association analysis to explore the candidate genes and molecular markers associated with various economic traits. The GWAS of the body weight at dl ,d28,d56 ,d80 ,dl00 of Beijing-You chicken using the Illumina 60K SNP Bead-chip was performed. The results showed that there were 9 SNPs reaching 5% Bonferroni genome-wide significance, which were significantly associated with body weight at d28 and dlOO, including LYRMl, LDB2 and other genes near these SNPs; There were 96 SNPs reaching the significance of "suggestive linkage", which were potentially associated with each indexes detected. The result revealed that these genes and SNPs laid a foundation for the development of techniques of marker assisted selection%旨在研究可卡因苯丙胺调控转录肽(Cocaine amphetamine regulated transcript,CART)对促卵泡素(Follicular stimulating hormone,FSH)诱导的猪卵巢卵泡颗粒细胞雌激素产生的影响.用Long-term培养方法在不同CART、FSH浓度下对猪卵巢卵泡颗粒细胞培养7d,在显微镜下观察各孔细胞生长情况并采集图像；采用双抗体夹心ABC-ELISA法测定各孔雌激素浓度.细胞培养168 h后:(1)在培养液不添加CART时,添加FSH孔中的雌激素浓度与不添加孔相比,显著提高；浓度为25 ng·mL-1时,雌激素浓度最高,为(17 295.57±184.03) pg·mL-1；(2) CART对体外培养的无FSH和FSH为5 ng·mL-1的猪卵巢卵泡颗粒细胞雌激素的产生没有影响；(3)当培养体系FSH浓度分别为25和50 ng·mL-1时,随着CART浓度的升高(0.01、0.1、1μmol·L-1),培养液雌激素浓度有下降的趋势,但各组间差异不显著(P＞0.05).但是,当FSH为25 ng·mL-1,CART为0.1和1 μmol·L-1时,与对照组相比,颗粒细胞雌激素的产生明显受到抑制(P＜0.05).但是,统计分析FSH和CART互作效应并不显著(P＞0.05),这一点需进一步试验证明；(4)当培养液添加50 ng·mL-1 FSH时,随着CART浓度的升高(0.01、0.1、1μmol·L-1),与对照组相比,雌激素浓度各组差异不显著(P＞0.05).猪卵巢卵泡颗粒细胞雌激素的产生需在FSH诱导下进行；当培养液FSH为25和50 ng·mL-1时,CART对FSH诱导的猪卵巢卵泡颗粒细胞雌激素的产生有抑制作用,但抑制效果并不显著.由此推断,并不像单胎的牛、羊,CART在猪卵泡发育过程中,可能并非是个主要的局部负调控因子.
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