Effect of the 17beta-Aminoestrogen Pentolame on Bone Mineral Levels in Ovariectomized Rats

摘要

Estrogens are fundamental to maintaining bone mineral balance, 17p-aminoestrogens produce low estrogenic effects through ERalpha and ERbeta receptors, however their effects on bone tissue are unknown. This work evaluates the effects of the 17P-aminoestrogen pentolame (AEP) and estradiol (E_2) on the mineral profile of rat femur. Six months after ovariectomy (Ovx) adult Wistar rats (200-250g) were treated every third day for 30 days with subcutaneous (s.c) injections of E_2 (1, 10, 100 ug/kg), AEP (1, 10, 100, 500 (ig/kg) or vehicle (propylenglycol; 1 ml/kg). After treatment, femur samples were prepared and Ca, P, Mg, Si, Fe, S, Na, K, and C concentration profiles were estimated using an X-ray analysis system coupled to an scanning electron microscope. Ovariectomy significantly decreased Ca, P, Mg and Si and increased Fe and S. Treatment with E_2 restored Ca, P, Mg, and Si to the control values and decreased Fe and S in a dose dependent manner. AEP restored the levels of Ca, P, Mg and Si at all doses administered. AEP increased the levels of Fe and restored S to the basal level. The other minerals showed great variability and no significant differences were detected. Our results indicate differential action of AEP related to E_2 in the restitution of bone mineral content.