盐酸帕罗西汀对卵巢切除大鼠学习记忆能力及血清雌激素水平的影响

摘要

目的：探讨抗抑郁剂盐酸帕罗西汀对卵巢切除大鼠学习记忆能力、血清雌激素水平及大鼠海马雌激素受体表达的影响。方法采用 SD 雌鼠双侧卵巢切除（OVX）作为雌激素波动引起的抑郁与认知障碍模型，用 Morris 水迷宫实验来测试大鼠的学习记忆功能。实验共分为正常对照组、OVX对照组、OVX 药物组。予5-羟色胺重摄取抑制剂类药物：盐酸帕罗西汀10 mg／（kg·d）干预4周。采用 ELISA 法测量SD 大鼠血清黄体生成素、卵泡刺激素、雌激素。免疫组化法检测大鼠海马雌激素受体α（ERα）和雌激素受体β（ERβ）的阳性细胞表达数。结果① Morris 水迷宫实验中，OVX对照组和 OVX 药物组的逃避潜伏期明显缩短，停留在平台时间百分比明显增加。② OVX 药物组的血清雌激素较OVX 对照组明显增加（P ＜0．05）。③药物组与非药物组的ERα、ERβ表达无明显差异。结论帕罗西汀增加OVX 大鼠血清雌激素水平和改善学习记忆能力，而对海马ERα、ERβ表达无明显影响。%Objective To observe whether paroxetine affects rats’learning and memory ability,serum estrogen and the expression of estrogen receptors in hippocampus.Methods The SD ovariectomized(OVX)rats were a-dopted as the model of estrogen's fluctuation leading to depressive symptoms and cognition disorder,treated with paroxetine by intragastric administration at 10 mg/(kg·d)for 4 weeks,and the sham rats as control group.Morris water maze was used to testify the effect of paroxetine on learning and memory ability.The level of serum luteinizing hormone,follicle-stimulating hormone,estrogen was measured by ELISA.Expressions of ERαand ERβin hipp-ocampal neurons were detected by immunohistochemistry.Results ① In Morris water maze experiment,compared to the control group,the escape latency of two drug-groups were obviously shortened;the time of staying in the plat-form significantly increased.② Serum estrogen of drug-group was significantly increased compared with the control groups (P <0.05).③ The ER expression between groups treated with paroxetine and the group without paroxetine in OVX rats had no difference.Conclusion Paroxetine increses serum estrogen levels and improves the learning and memory ability,and has no significant effect on ER expression in hippocampal neurons.