猪肺炎支原体感染早期诱导猪呼吸道产生多种特异性IgA抗体分泌规律研究

摘要

为了研究猪感染猪肺炎支原体早期,呼吸道针对病原不同功能蛋白分泌特异性IgA抗体的差异与规律,本研究通过已建立的猪肺炎支原体各蛋白的特异性IgA间接ELISA方法,分别检测7头试验猪在感染后第1、2、4、6、8、12、16和21天鼻拭子中抗P36、P46、P97R1的特异性IgA抗体滴度,并经过样品总蛋白浓度校准后,比较3种特异性IgA抗体在感染后21d内的分泌规律.结果显示,在感染后第6天,呼吸道中抗猪肺炎支原体P36、P46、P97R1的3种特异性IgA抗体分泌量均开始上升,于感染后第12天达到高峰,并可持续到第21天,且同1d内3种特异性IgA抗体之间的分泌量差异不显著(P＞0.05).本研究说明在猪肺炎支原体感染的早期阶段,P36、P46、P97R1蛋白均可诱发猪呼吸道产生特异性IgA抗体,且分泌量与分泌规律较为相似,差异不显著.%This experiment was conducted to study the initial secretive features of specific IgA antibodies against P36,P46 and P97R1 proteins produced in the respiratory tract after the pigs were affected with Mycoplasma hyopneumoniae (Mhp).Total seven pigs were infected with Mhp strain.The nasal swabs were collected at the 1st,2nd,4th,6th,8th,12th,16th and 21st day post inoculation (dpi) to detect specific IgA titers against P36,P46,P97R1 proteins by the three established indirect enzyme-linked immunosorbent assays (ELISA).The IgA titers were normalized by the total protein concentration of nasal swab samples.The results showed that levels of three sorts of specific IgA start to rise at the 6th dpi and reach the peak at the 12th dpi.The high levels could be kept until to end of assay (the 21st dpi).The secretive features of three specific IgA antibodies were consistent as well as the secretive amounts on the same day (P＞0.05).In conclusion,P36,P46 and P97R1 proteins could induce the specific IgA antibody in the respiratory tract during the early stage of infection with the same trends and secretive amounts.