硒化党参多糖和大蒜多糖协同增强鸡外周血淋巴细胞和新城疫疫苗的免疫功效

摘要

In order to validate the synergistic immune-enhancing action of selenizing Codonopsis pilosula polysaccharide (sCPP) and garlic polysaccharide (GP) and the efficacy of their compound sCPP-GP and offer theoretical evidence for developing new-type immunopotentiators,the immune-enhancing activities of these three kinds of polysaccharide were compared.In vitro test,the effects of three polysaccharides on chicken peripheral lymphocytes proliferation and mRNA transcription of IL-2 and IFN-γ were determined respectively by MTT method and Real-time quantitative PCR technique.In vivo test,the chickens were injected with three polysaccharides when vaccination of Newcastle disease vaccine,the dynamic changes of chicken peripheral lymphocytes proliferation,serum HI antibody titer,IL-2 contents and IFN-γ contents were determined.The results of in vitro test showed that the three polysaccharides at appropriate concentrations could significantly promote lymphocytes proliferation and mRNA transcription of IL-2 and IFN-γ,and the effects of compound were significantly stronger than those of two constituents;The results of in vivo test showed that at four time points after vaccination three polysaccharides could promote lymphocytes proliferation and enhance serum HI antibody titer and contents of IL-2 and IFN-γ at different degrees,and the efficacy of compound were significantly stronger than those of constituents.These results indicate that sCPP and GP can synergistically enhance the immunological efficacy of chicken peripheral lymphocyte and Newcastle disease vaccine,sCPP-GP compound's action is stronger than its two constituents,and can be a candidate of new-type immunopotentiators.%为了验证硒化党参多糖(selenizing Codonopsis pilosula polysaccharide,sCPP)和大蒜多糖(garlic polysaccharide,GP)的协同增强免疫作用及其复方硒化党参多糖-大蒜多糖(sCPP-GP)的功效,为研制新型免疫增强剂提供理论依据,比较了这3种多糖的增强免疫活性.体外试验,用MTT法和RT-PCR法测定了3种多糖对鸡外周血淋巴细胞的增殖及其IL-2和IFN-γ mRNA转录的影响;体内试验,将3种多糖配合新城疫疫苗免疫雏鸡,测定鸡外周血淋巴细胞增殖、血清HI抗体效价、血清IL-2和IFN-γ含量的动态变化.体外试验结果显示,3种多糖在合适的浓度均能显著促进淋巴细胞增殖及IL-2和IFN-γ mRNA的转录,复方的作用显著强于两个组分药;体内试验结果显示,在免疫后4个时间点,3种多糖均能不同程度地促进T淋巴细胞增殖,提高血清HI抗体效价、提高血清IL-2和IFN-γ的含量,复方的作用显著强于两个组分药.研究结果表明,sCPP和GP能协同增强鸡外周血淋巴细胞和新城疫疫苗的免疫功效,复方sCPP-GP的作用显著强于两个组分药,可以作为新型免疫增强剂的候选方.