In order to reveal the imprinting status of Gab1 (Grb2-associated binding protein 1) and Sfmbt2 (Scm-like with four mbt domains 2) in different tissues and placenta of bovine,in this study,we analyzed the imprinting status of Gab1 and Sfmbt2 in 7 bovine tissues (heart,liver,spleen,lung,kidney,muscle and fat) and placenta by direct sequencing the RT-PCR products based on single nucleotide polymorphisms.The result displayed that Gab1 and Sfmbt2 were all expressed in 7 tissues and placenta.We discovered that Gab1 and Sfmbt2 were bi-allelic expressed by comparing sequence chromatograms of RT-PCR products and genomic PCR products at single nucleotide polymorphisms sites,suggesting that Gab1 and Sfmbt2 were not imprinted in adult cattle tissues and placenta.%旨在揭示Gab1(Grb2-associated binding protein 1)和Sfmbt2 (Scm-like with four mbt domains 2)基因在牛不同组织以及胎盘中的印记状态,本研究应用基于单核苷酸多态性(Single nucleotide polymorphisms,SNP)的RT-PCR (Reverse transcription-PCR)产物直接测序方法对Gab1和Sfmbt2基因在牛7个组织(心、肝、脾、肺、肾、肌肉和脂肪)以及胎盘中的印记状态进行分析.结果显示,在被分析的7个组织和胎盘中均检测到Gab1和Sfmbt2基因的表达.通过比较杂合子牛基因组PCR扩增产物与RT-PCR扩增产物在SNP位点的测序峰图,发现Gab1和Smbt2在被检测的7个组织和胎盘中均为双等位基因表达,说明Gab1和Smbt2在牛的组织和胎盘中是非印记的.
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