石榴皮多酚提取物对亚急性瘤胃酸中毒相关有害因子的影响

摘要

This study established a in vitro subacute ruminal acidosis ( SARA) model to evaluate the effects of different supplemental levels of pomegranate peel polyphenols extract (PPPE) on related harmful factors of subacute ruminal acidosis. Single factor design was used for the trial, and 3 supplemental levels of PPPE were design, which were 0, 4. 5% and 9.0% of fermentation substrate, respectively. Each supplemental level had 5 replicates. The results showed as follows; with prolonged fermentation time, the pH of fermentation broth was gradually decreased. The PPPE supplementation could retard the pH decreased, and the pH of fermentation broth at the 6th, 12th and 18th h in the group containing 4. 5% PPPE was significantly higher than that in the group containing 9.0% PPPE (P <0.05). The contents of endotoxin and histamine in the fermentation broth were significantly decreased by PPPE supplementation (P <0.05), and showed a dose-dependant effect. After supplemented PPPE, the volatile fatty acid contents were all decreased to different extent, and significant effects were found in some time points (P <0. 05). The results indicate that PPPE supplementation can effectively relieve SARA, and the 4. 5% supplemental level has a better effect.%本文通过建立体外亚急性瘤胃酸中毒(subacute ruminal acidosis,SARA)模型,研究不同添加水平的石榴皮多酚提取物(pomegranate peel polyphenol extract,PPPE)对SARA相关有害因子的影响.试验采用单因素试验设计,PPPE设3个添加水平,即分别为发酵底物质量的0、4.5％和9.0％,每个水平设5个重复.结果表明:随发酵时间的延长,各组发酵液的pH逐渐下降,添加PPPE可减缓pH的下降,且PPPE添加量为4.5％的组发酵液pH在6、12、18 h时显著高于添加量为9.0％的组(P＜0.05).添加PPPE显著降低发酵液中内毒素和组胺含量(P＜0.05),且呈现剂量效应.添加PPPE后,发酵液中各挥发性脂肪酸含量均有不同程度地降低,并在部分时间点达到显著水平(P＜0.05).在本试验条件下,添加PPPE有助于缓解SARA,且以4.5％的添加量效果较好.