仙台病毒核酸快速检测方法的建立和应用

摘要

仙台病毒（Sendai virus，SeV）是一种常见的可引起啮齿类动物呼吸道疾病的病原，感染迅速，并且隐性感染率高，一旦感染较难从鼠群中清除，从而影响动物健康。为满足对入境噬齿类实验动物和野生动物仙台病毒快速检测的需要，本研究针对SeV编码基质蛋白和融合糖蛋白基因的保守序列设计引物和荧光探针，建立了仙台病毒RT-PCR和Real-time RT-PCR检测方法。将建立的方法应用于仙台病毒感染鼠不同临床样品和组织培养物的检测，证实两种核酸检测方法具有良好的特异性、灵敏性和稳定性，适合应用于出入境口岸实验和野生噬齿类动物仙台病毒疫情的快速检测。%Sendai virus (SeV) is one of common pathogens causing respiratory disease among rodent animals, which is characterized by rapid infection and high recessive infection rates. Therefore, SeV infection is difficult to eliminate from affected animals and might interfere with animal experiments. In order to meet requirements for rapid quarantine for rodent animals, RT-PCR and Real-time RT-PCR methods were developed using TaqMan probe to detect SeV. Both methods were applied to test clinical samples collected from SeV infected mice and cell cultures. The results showed that RT-PCR and Real-time RT-PCR methods were specific, sensitive and reproducible thus both methods were suitable for diagnosis of SeV infection in rodent animals.