山东省血清4型禽腺病毒的分离鉴定

摘要

Ten virus isolates were obtained from the diseased chickens with pericardial effusion in Shandong province. These isolates were identifi ed as serotype 4 Fowl adenovirus through PCR detection and serotyping. The clinical signs and lesions were reproduced in SPF chickens via experimental infection with the isolated viruses. The inactivated vaccine prepared from the obtained isolate prevented the reoccurrence and injection of immune serum provided good therapeutic treatment for diseased chickens.%近期从山东省许多地区爆发的以心包积液为主要病变特征的发病鸡中分离到10株病毒，经PCR检测和血清型鉴定，确定为血清4型禽腺病毒。SPF鸡回归试验证实，分离到的病毒能完全复制出与临床自然发病鸡相同的症状与病变。用该病毒制备的灭活疫苗具有良好的免疫保护作用，制作的抗体也具有良好的治疗效果。