2型和11型鸭疫里默氏菌二价灭活苗的研制

摘要

为研制血清2型和11型鸭疫里默氏菌（Riemerella anatipestifer，RA）二价灭活疫苗，以RA1325株（2型）和RA1229株（11型）为菌种，经培养、灭活、浓缩，混合后制成二价灭活油乳剂疫苗。将疫苗皮下接种7日龄番鸭，免疫后7、14、21、56、63、70、77 d进行攻菌试验，测定疫苗的免疫原性。免疫后每隔7 d用ELISA方法测定血清中的抗体。结果表明，疫苗免疫后14 d产生良好保护，保护率超过80%，免疫持续期可达60 d。免疫后14~63 d抗体效价处于较高水平，说明研制的二价灭活疫苗能对血清2型和11型RA引起的鸭疫里默氏菌病产生良好的预防效果。%The purpose of the present study was to develop an inactivated bivalentRiemerella anatipestifer vaccine containing serotypes 2 and 11.Riemerella anatipestifer strains RA1325 (serotype 2) andRA1229 (serotype 11) were cultured, inactivated and concentrated separately and mixed before formulation of a bivalent vaccine. To determine the immunogenicity of the vaccine, seven-day-old Muscovy ducks were subcutaneously vaccinated and then challenged with the live vaccine strains at days 7, 14, 21, 56, 63, 70 and 77 post challenge. Antibodies in serum samples were determined in ELISA every 7 days post vaccination. The results showed that the protection rate was over 80% at day 14 post challenge. The immunity duration of the vaccine was 60 days. The titers of antibodies during 14 to 63 days post vaccination were at a high level. The preliminary results indicated that this bivalent vaccine might be used to prevent serotypes 2 and 11Riemerella anatipestifer infection in ducks.