鸡源锌指蛋白ZEB1基因解析及其在新城疫病毒感染中的作用研究

摘要

Zinc protein ZEB1(zinc finger E-box binding homeobox 1) is a transcription repressor, which is highly conserved in different species. To determine the correlation of ZEB1 with Newcastle disease virus(NDV) infection, the gene of chicken ZEB1 was PCR-amplified from DF-1 cells and used for phylogenetic and bioinformatic structure analysis. The expression of ZEB1 in NDV-infected DF1 cells, CEF cells and SPF chickens were examined in Real-time PCR. The results showed that NDV infection introduced high expression of chicken ZEB1, suggesting ZEB1 protein might play a role in the life cycle of NDV. Nevertheless, higher expression of ZEB1 did not affect the growth and protein expression of NDV. Therefore, the biological role of Zinc protein ZEB1 need to be further studied.%锌指蛋白ZEB1(zinc finger E-box binding homeobox 1)是一种在不同物种中相对保守的转录因子,尤其在发生肿瘤的组织中具有较高的表达水平.为了证实禽类锌指蛋白ZEB1在新城疫病毒(Newcastle disease virus,NDV)感染中的作用,本研究扩增获得了鸡源ZEB1的基因序列,并通过生物信息学软件对不同物种的ZEB1基因进行了同源性比对.通过Real-time PCR技术测定NDV感染DF-1细胞、CEF细胞以及SPF鸡后ZEB1的表达量变化,证实NDV感染可使细胞中的ZEB1含量显著升高.但是在细胞中过表达ZEB1对NDV的增殖和蛋白表达没有明显的影响,其生物学意义有待进一步研究.