副猪嗜血杆菌ompP2基因的克隆鉴定及序列分析

摘要

本文用PCR技术对华南地区分离的15株不同血清型副猪嗜血杆菌菌株和2株国际参考菌株的外膜蛋白编码基因ompP2基因进行克隆鉴定,并以CLU STALS1和PHYLIP-3.68软件将ompP2基因序列进行比对和遗传进化分析.l7株副猪嗜血杆菌菌株均能扩出ompP2基因,克隆测序结果发现o mp P2基因长度为1077～1092 bp,其中菌株H37、H38和H49中P2存在两段34～39个氨基酸的保守插入序列.β折叠预测结果显示H37、H38和H49比其他菌株多一个环结构.遗传进化分析显示H37、H38和H49 3株含有插入片段的菌株聚为一群,与其他菌株遗传距离相对较远.上述结果为进一步验证ompP2蛋白的功能及相关研究奠定了基础.%We used PCR to determine the ompP2 gene of 15 H.parasuis local isolates and 2 reference strains.Then we sequenced the ompP2 gene of H.parasuis.All ompP2 gene sequence from H.parasuis,were analyzed for phylogenetic relationship.An approximately 1.1 kb fragment was obtained from the genomic DNA of 17 H.parasuis strains.Sequence analysis showed that the size of the ompP2 gene was difference,and had two conserved sequences of 34 ～ 39 amino acids in strains H37,H38 and H49.Beta folding prediction showed which had more than one ring structure and relatively far distance in strains H37,H38 and H49 than other strains.These results laid the foundation for further verification of the function and related research ofompP2 protein.