Objective To investigate the mechanism of Longchang granule for treatment of benign prostatic hyperplasia. Methods Rat model with prostate hyperplasia was established by testosterone propionate injection after it was first castrated. The rat models were killed after being treated with administration of drug by gastrogavage for 30 days, and then prostate tissue were collected for analysis. The wet weight of prostate tissue was measured and the expressions of bcl-2 gene in prostate tissue under the influence of Longchang granule and Longbishu were detected by RV 2-steps method. Results The verage wet weight of prostate was 0.61±0.03(blank group), 0.95±0.04 (model group), 0.73±0.02(Longbishu group), 0.80±0.05 (low dose Longchang granule group, 0.78±0.07(normal dose Longchang granule group)and 0.68±0.03( high dose Longchang granule group) respectively. There was a significant difference in wet weight of prostate between Longchang granule group and model group(P<0.05). Prostate index of different groups was 0.143±0.006, 0.226±0.008, 0.172±0.004, 0.199±0.012, 0.181±0.010 and 0.168±0.003 respectively. There was an obvious difference in prostate index between Longchang granule group and model group(P<0.05).The expression of Bcl-2 in benign prostatic hyperplasia rats treated with Longchang granule was 0.089±0.010,0.131±0.005,0.093±0.015, 0.109±0.001,0.097±0.003 and 0.091±0.003 respectively .there exsited a significant difference in Bcl-2 expression between Longchang granule group and model group(P<0.05). Conclusion Longchang grandule can effectively reduce the wet weight of prostate and relieve pathological changes. The possible mechanism was associated with downregulation of bcl-2 expressions in prostatic tissue and further facilitate the apoptosis of prostate cells.%目的 探讨癃畅颗粒对治疗前列腺增牛作用机制.方法 采用大鼠去势后注射丙酸睾酮致前列腺增生法造模,灌胃给药后30d处死.摘取前列腺组织并测量湿重,采用PV两步法对癃畅颗粒和癃闭舒干预的大鼠前列腺增生组织进行bcl-2基因检测及组织病理学观察.结果 前列腺湿重:空白组(0.61±0.03)g,模型组(0.95±0.04)g;癃闭舒组(0.73±0.02)g;癃畅颗粒低剂量组(简称癃畅低组)(0.80±0.05)g,癃畅颗粒中剂量组(简称癃畅中组)(0.78±0.07)g;癃畅颗粒高剂量组(简称癃畅高组)(0.68±0.03)g;与模犁组比较P<0.05.前列腺指数:正常组、癃闭舒组、癃畅低组、癃畅中组和癃畅高量组分别为:0.143±0.006,0.226±0.008,0.172±0.004,0.199±0.012,0.181±0.010和0.168±0.003;分别与模型组比较P<0.05.癃畅颗粒对BPH大鼠前列腺上皮细胞bcl-2基因表达影响(平均光密度):正常组、癃闭舒组、癃畅低组、癃畅中组和癃畅高组分别为:0.089±0.010,0.131±0.005,0.093±0.015,0.109±0.001,0.097±0.003和0.091±0.003;与模型组比较P<0.05.结论 癃畅颗粒能够有效缩小模型大鼠前列腺湿重,减轻病理变化,其作用机制可能为下调大鼠前列腺bcl-2基因表达,促进前列腺细胞凋亡.
展开▼
