小鼠脑缺血后小胶质细胞活化及细胞周期蛋白的表达规律

摘要

Objective:To explore the pattern of microglia activation and expression of cyclin D1 and cyclin-dependent kinase 4 (CDK4) after cerebral ischemia in mice. Methods:The focal cerebral ischemia/reperfusion(I/R) model in mouse was made by using middle cerebral artery occlusion. Mice were randomly divided into the following groups: sham group, occluded for 40 min and reperfusion for 3 h, 5 h, 12 h, 1 d, 3 d and 5 d. The histochemical staining was used to observe the activation of microglia by IB4 after ischemia. The immunohistochemical staining was used to study the distribution of cyclin D1 and CDK4. Results: Activating microglia increased after ischemia reperfusion 12 h. Activating microglia significantly increased 3 d after ischemia reperfusion, and microglia cells continues increased 5 d after ischemia reperfusion. Cyclin D1 expression was peaked at 1 d after reperfusion, and cyclin D1 expression decreased from 3 d. CDK4 immunoreactivity increased to the peak after ischemia reperfusion 3 d, and CDK4 expression decreased from 5 d. Conclusion : Microglia activation and expression of cyclin D1 and CDK4 is not synchronized, while cyclin D1 and CDK4 can promote microglia activation.%目的:探讨小鼠脑缺血后小胶质细胞活化及细胞周期蛋白D1与细胞周期蛋白依赖蛋白激酶4(CDK4)的表达规律.方法:采用线栓法制作小鼠脑缺血/再灌注(I/R)模型,动物随机分为假手术组和缺血组,缺血组在缺血40 min再分为再灌注3h、5h、12h、1d、3d和5d组,组织化学显色观察小胶质细胞活化规律,免疫组织化学显色观察细胞周期蛋白D1和CDK4的表达.结果:缺血再灌注12h后活化的小胶质细胞增多,缺血再灌注3d后活化的小胶质细胞显著增多,5d后活化的小胶质细胞持续增加.细胞周期蛋白D1表达缺血再灌注1d达高峰,3d和5d时细胞周期蛋白D1表达下降.CDK4表达在缺血再灌注3d达高峰,5d后开始下降.结论:细胞周期蛋白D1和CDK4与小胶质细胞的活化不同步,细胞周期蛋白D1和CDK4可促进小胶质细胞的活化.