. PU.1 knockout murine microglia cell line and uses thereof

摘要

The present invention relates to a mouse microglia cell line of PU.1 (spleen focus forming virus (SFFV) proviral integration oncogene, Spi1; Sfpi1) knockout mouse and a method for screening a substance for preventing or treating inflammatory neurodegenerative diseases using the same. More particularly, the present invention relates to a method for producing a PU.1 knockout mouse microglia cell line using a guide RNA for recognizing a PU.1 gene, a guide RNA vector containing the same, a guide RNA vector containing the guide RNA, The PU.1 knockout mouse microglial cell line prepared by the above method, a screening method of a substance for the prophylaxis or treatment of inflammatory neurodegenerative diseases using the same, and a method for screening for inflammatory neurodegenerative diseases including the PU.1 knockout mouse microglial cell line Or a therapeutic substance screening composition. The PU.1 gene knockout mouse microglial cell line prepared using the guide RNA of the present invention can be used not only for the functional study of the PU.1 gene but also for the functional study of the PU.1 gene in the microglial cells And thus can be useful for screening therapeutic agents for neurodegenerative diseases.