气相色谱电子捕获检测法测定人尿中α羟基三唑仑及α羟基阿普唑仑

摘要

建立了尿中三唑仑的代谢物α羟基三唑仑和阿普唑rn仑的代谢物α羟基阿普唑仑的气相色谱电子捕获检测法。代谢物用N，O双三甲基硅烷基三氟乙酰胺（BSTFA）进行三甲基硅烷衍生化后进行检测。尿中代谢物的检出限约为1μg/L（S/N=3），至少可检出口服治疗量药物人体24h之内排泄尿中的代谢物，满足麻醉后犯罪案件中药物检验的要求。%A method was developed to assay α-Hydroxyltriazolam and α-Hydroxyalprazolam,　which are the major metabolites of triazolam and alprazolam respectively,in human urine.　After addition of 2-hydroxyflurazepam　(interal standard) and hydrolysis with β-glucuronidase, the hydroxy-metabolites were extracted with hexane-dichloromethane (1∶1) at pH　10.8, then were derivated with (BSTFA). The analysis was performed on a HP-5 capillary column with electron-capture detector.The detection limits of analysis in urine were about 1μg/L.The method was successfully applied to urine specimens collected from healthy human volunteers who ingested 0.5 mg of triazolam or 0.8 mg alprazolam. The method was enough sensitive to assay urine specimen excreted at 24 h by volunteers after taking the medicine.