金属离子存在下5-氟尿嘧啶与脱氧核糖核酸、血清白蛋白的相互作用

摘要

紫外光谱、荧光光谱、圆二色谱法等方法研究了抗癌药物5-氟尿嘧啶(5-fluorouracil,5-FU)与DNA、血清蛋白的相互作用及某些二价金属离子对其作用的影响。求得了5-FU与HSA、BSA的结合常数。%The interaction of 5-fluorouracil (5-FU) with deoxyribonucleic acid (DNA) and serum albumin and the influence of some metal ions on the interaction have been studied by using spectral methods of UV-absorption,fluorescence and circular dichroism. It was shown that the interaction of 5-FU with DNA and serum albumin was effected by some metal ions. The binding costant of 5-FU with serum albumin was found to be KHSA = 4.38 × 103(mol/L)-1 and KBSA =4.95 × 103(mol/L)-1 and the distance between the bound 5-FU and the tryptophan residue was also determined to be rHSA = 3.53 nm, rBSA = 3.51 nm.