A method for the simultaneous determination of seven adulterants including fenfluramine, pseudoephedrine, norpseudoephedrine, amfepramone, sibutramine, sildenafil and strychnine in slimming functional foods has been established. A mixture of 20 mmol/L sodium tetraborate-10 mmol/L SDS-5% acetonitrile was used as running buffer (pH 9. 0). The samples were analyzed by high performance capillary elctrophoresis under conditions of 17 kV; 25℃ with the detection wavelength of 195 nm. The upper limits of linear ranges were all 100 mg/L with linear correlation coefficients greater than 0. 998. The RSDs were 4. 5%-7. 9% (n = 7). The average recoveries were 79. 6% -112. 0%. The detection limits (S/N=3) were 0. 16 - 0. 65 mg/L. The method is simple, fast, sensitive, accurate, low cost and has been successfully applied to the determination of the 7 target chemicals in the slimming functional foods.%建立了同时测定减肥类功能食品中违禁成分芬氟拉明、伪麻黄碱、去甲伪麻黄碱、安非拉酮、西布曲明、西地那非和士的宁的高效毛细管电泳分析方法.以20 mmol/L硼砂-10 mmol/L SDS-5％乙腈溶液(pH 9.0)为运行缓冲液,在25℃,17 kV条件下测定,检测波长为195 nm.各待测物线性范围上限均为100 mg/L,线性相关系数均大于0.998,相对标准偏差为4.5％～7.9％(n=7)；平均加标回收率为79.6％～112.0％；检出限(S/N=3)为0.16～0.65 mg/L.本方法简便快速、灵敏准确、分析成本低,已成功应用于功能食品中上述减肥类违禁添加成分的测定.
展开▼
