A method of ultra performance liquid chromatography-tandem mass spectrometry (UPLCMS/MS) has been developed for the determination of albendzole(ABZ) and its metabolites, alben-dazole sulfoxide (ABZSO), albendazole sulfone (ABZSO2) and albendazole aminosulfone (ABZ-2-NH2SO2) in grass carp meat. The samples were extracted with alkaline ethyl acetate and cleaned up by n-hexane. Chromatographic separation was achieved by UPLC and the detection was performed by tandem mass spectrometry using deuterated isotope internal standard. The UPLC-MS/MS analysis was rapid and can be achieved in less than 4 min. In the range of 0.1-10 μg/kg, the analytes were in good linearity, and the correlation coefficients were 0. 9991-0. 9996. The recoveries of ABZ, ABZSO, ABZSO2 and ABZ-2-NH2SO2 spiked at levels of 0. 5-5. 0 μg/kg, were 98. 4％ - 102. 6％, 97.1％-103.4％, 98.8％-103.7％, 101. 1％-104.2％, the limit of quantity (LOQ) were 0.2, 0.1, 0.2, 0.1 μg/kg, respectively. The Intra-day RSD were less than 4.8％, and Inter-day RSD was less than 3.0％. The UHPLC-MS/MS method is rapid, sensitive, reproducible and suitable for the determination of ABZ and its metabolites%建立了同时测定草鱼肉中阿苯达唑及其3种代谢物阿苯达唑亚砜、阿苯达唑砜、阿苯达唑-2-氨基砜的超高效液相色谱-串联质谱(UPLC-MS/MS)分析方法.草鱼肉样品通过碱性乙酸乙酯提取,正己烷净化,超高效液相色谱分离,串联质谱检测,氘代同位素内标定量.本方法分析时间短,在4min内即可完成4种被分析物的液质联用分析.本方法在0.1～10μg/kg范围内各物质线性良好,线性系数在0.9991～0.9996之间.阿苯达唑、阿苯达唑亚砜、阿苯达唑砜、阿苯达唑-2-氨基砜在0.5～5.0μg/kg添加水平下的平均回收率分别为98.4%～102.6%,97.1%～103.4%,98.8%～103.7%和101.1%～104.2%检出限分别为0.2,0.1,0.2和0.1μg/kg;日内精密度小于4.77%,日间精密度小于3.03%.本方法为阿苯达唑及其代谢物的检测及监控提供了一种快速、灵敏度高、重现性好的定量分析方法.
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