A method has been developed for the determination of nine sensitizing disperse dyes in dyeing wastewater by solid phase extraction-ultra performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (SPE-UPLC-ESI-MS/MS). The samples were extracted and purified by solid phase extraction. UPLC separation was performed on an ACQUITY UPICTM BEH C18 column utilizing a gradient elution program of acetonitrile and acidified water (containing 2％ acetonitrile, 0.2％ formic acid, 0. 005 mol/L ammonium, pH 2.7) as the mobile phase. Identification and quantification were achieved by UPLC-ESI-MS/MS in positive mode and multiple reactions monitoring. Good linearity was observed in the range of 5—1000 μg/L with correlation coefficients in the range of 0.9929—0. 9999, limit of detection (LOD) for the nine sensitizing dyes was in the ranges of 0.05—2.48 μg/L. The recoveries ranged from 70.0％ to 109％. The application of this method was demonstrated by analyzing sensitizing disperse dyes＇ residual concentration in five dyeing enterprise wastewater. Disperse blue 106 and disperse blue 35 were not detected. The detect concentration of other seven sensitizing disperse dyes was more than 40 tg/L.%建立了同相萃取-超高效液相色谱-电喷雾串联三四级杆质谱(SPE-UPLC-ESI-MS/MS)联用技术分析印染废水中9种致敏性分散染料的方法.样品经SPE提取和净化后,采用Waters ACOUITY UPLCTMBEHC<,18色谱柱,乙腈和酸化水(2%乙腈,0.2%甲酸,0.005 m01/L甲酸铵,pH 2.7)作为流动相进行梯度洗脱,电喷雾离子源电离,正离子多反应监测模式进行定性和定量分析.9种致敏性分散染料在5～1000 μg/L浓度范嗣内线性良好.相关系数0.9929～0.9999,方法的检出限为0.05～2.48 μg/L;回收率为70.0%～109.0%.应用本方法检测5家印染企业废水中的致敏性分散染料残留浓度,分散蓝106和分散蓝35未检出,其它7种致敏性分散染料的检出浓度均高于40 μg/L.
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