IL-17、TNF-α在胶原诱导的关节炎大鼠模型外周血的表达

摘要

目的 探讨白细胞介素-17(interleukin-17,IL-17)、肿瘤坏死因子-α(tumour necrosis factor-α,TNF-α)在胶原诱导的关节炎(collagen-induced arthritis,CIA)大鼠模型的表达特点.方法 建立CIA大鼠模型,以X线片及组织病理学证实造模成功;用ELISA分析CIA大鼠模型血清炎性细胞因子IL-17、TNF-α的水平.结果 与对照组比较,CIA大鼠模型外周血IL-17、TNF-α水平明显升高(P<0.05).结论 在成功建立的CIA大鼠模型中,IL-17、TNF-α大量产生,提示IL-17可能参与了类风湿关节炎(rheumatoid arthritis,RA)的发病过程.%Objective To detect the expression characteristics of IL-17 and TNF-α in collagen-induced arthritis (CIA) rats model. Methods To establish the collagen-induced arthritis rats model was established, then the serum level of IL-17 and TNF-α were determined by ELISA, and the degree of pathogenicity as well as the pathological characteristics were assayed by histopathological techniques and joint roentgenography. Results In CIA model the level of IL-17 and TNF-α measured by ELISA were significantly higher than those of the normal group (P < 0.05). As demonstrated by histopathology and roentgenography, typical arthritis pathology with chronic proliferative synovitis and secondarily destroyed articular cartilage and bone was found. Conclusion The animal model with CIA was successfully established in this experi- ment. The serum level of IL-17 and TNF-α increased significantly, indicating that IL-17 might involved in the pathogenesis of rheumatoid arthritis.u0000ila